Transcript (Accesion No.) |
Primers/probe sequences |
Kv11.1 (U87513) |
forward: 5'-GCTCATTGGCTCCCTCATG-3' |
Kv7.1 (AJ291316) |
forward: 5'-AGGAGCTGATCACCA-3' |
Kv4.2 (AF493547) |
forward: 5'-CAGATCAGATGTGTGGAGAGAACA-3' |
Kv4.3 (AF085170) |
forward: 5'-ATTGATGAGCAGATG-3' |
18S rRNA (EF060919) |
forward: 5'-GAGGGAGCCTGAGAAACGG-3' |
HIV-1 PR D25K |
forward: 5'-GAAGCTCTGCTGAAGACTGGCGCTGACGATACC-3' |
HIV-1 PR D25R |
forward: 5'-GAAGCTCTGCTGCGTACTGGCGCTGACGATACC-3' |
HIV-1 PR D25W |
forward: 5'-GAAGCTCTGCTGTGGACTGGCGCTGACGATACCG-3' |
HIV-1 PR G49E |
forward: 5'-CCAAAAATGATAGGGGAAATTGGAGGTTTTATCAAAG-3' |
HIV-1 PR G49W/I50W |
forward: 5'-CCAAAAATGATAGGGTGGTGGGGAGGTTTTATCAAAG-3' |
HIV-1 PR D25R/G49E/I50R |
forward: 5'-CCAAAAATGATAGGGGAAAGAGGAGGTTTTATCAAAGTAAGAC-3' |
CMV promoter |
forward: 5'-GCGGGATCCGGAGTTCCGCGTTACATAAC-3' |
pMDLg/pRRE D25K |
forward: 5'-GGCAATTAAAGGAAGCTCTATTAAAGACAGGAGCAGATGATACAG-3' |
pMDLg/pRRE D25R |
forward: 5'-GGCAATTAAAGGAAGCTCTATTACGTACAGGAGCAGATGATACAG-3' |
pMDLg/pRRE dN |
A (outer, forward): 5'-GGCCCCTAGGAAAAAGGGCTGTTGGAAATG-3' |
pMDLg/pRRE dC |
A (outer, forward): 5'-GGCCCCTAGGAAAAAGGGCTGTTGGAAATG-3' |
PAI-1 |
forward: 5'- ATCTTCGTCCAGCGG -3' probe: 5'- FAM-ATCTGAAGCTGGTCCAGGGCTTCA-TAMRA -3' reverse : 5'- GCCTGAAGAAGTGGG -3' |
PAI-2 |
forward: 5'- GCTCTGCAATCAATGCA -3' probe: 5'- FAM-ACAGGGGATTATTTACTGGAAAGTGTCA- TAMRA -3' reverse: 5'- GACTTCTCACCAAACAG -3' |
uPA |
forward: 5'- GCGCTGACACGCTTGCT – 3’ probe: 5'- FAM-ACCACAACGACATTGCCTTGCTGAAGA-TAMRA – 3’ reverse: 5'- CACCTGCCCTCCTTGGAA – 3’ |
tPA |
forward: 5’- GTGCCCCGAAGGATTTG – 3’ probe: 5'- FAM-ACGTGGCCCTGGTATCTATTTCACAGCA-TAMRA – 3’ reverse : 5'- ATGCCCTGGTCCTCGTA – 3’ |
HIV-1 CA W23A |
forward: 5’-CAC CTA GAA CTT TAA ATG CAG CGG TAA AAG TAG TAG AAG AGA AGG-3’ reverse: 5’- CCT TCT CTT CTA CTA CTT TTA CCG CTG CAT TTA AAG TTC TAG GTG-3’ |
HIV-1 CA A77P |
forward: 5’-GAC CAT CAA TGA GGA ACC TGC AGA ATG GGA TAG AG-3’ reverse: 5’-CTC TAT CCC ATT CTG CAG GTT CCT CAT TGA TGG TC-3’ |
HIV-1 CA A78V |
forward: 5’-CCA TCA ATG AGG AAG CTG TAG AAT GGG ATA GAG TAC-3’ reverse: 5’-GTA CTC TAT CCC ATT CTA CAG CTT CCT CAT TGA TGG-3’ |
HIV-1 CA L189F |
forward: 5’-GGA TGA CAG AAA CCT TCT TGG TCC AAA ATG CGA AC-3’ reverse: 5’-GTT CGC ATT TTG GAC CAA GAA GGT TTC TGT CAT CC-3’ |
HIV-1 CA L189I |
forward: 5’-AAT TGG ATG ACA GAA ACC ATC TTG GTC CAA AAT GCG AAC-3’ reverse: 5’-GTT CGC ATT TTG GAC CAA GAT GGT TTC TGT CAT CCA ATT-3’ |
HIV-1 CA L189P |
forward: 5’-AAT TGG ATG ACA GAA ACC CCG TTG GTC CAA AAT GCG AAC-3’ reverse: 5’-GTT CGC ATT TTG GAC CAA CGG GGT TTC TGT CAT CCA ATT-3’ |
HIV-2 PR D25N Asp25 - Asn25 |
5’ GAAGTTTTGTTAAATACGGGAGCTGAC 3’ |
HIV-2 Tat Y44A Tyr44 - Ala44 (GCC) |
5’CTCTCTCAGCTAGCCCGACCCCTAGAAAC 3' |
HIV-2 Rev H73R H73 - R73 |
5' CCAGACTATACAGCGTCTGCAGGGACTTAC 3' |
HIV-2 Vif S144A Ser144 - Ala144 |
5' GAGCCCAGGTACCGGCACTTCAATTTCTG 3' |
HIV-2 Vpr R78A Arg78 - Ala78 |
5' TTCACGCACTTCGCAGCAGGATGTGGCC 3' |
HIV-2Vpx K68A, R70A Lys68 - Ala68 Arg70 – Ala70 |
5' GGGATGTCAGAAAGTTACACAGCGTATAGATATTTGTGCATAATACAG 3' 5' GTTACACAGCGTATGCATATTTGTGCATAATACAG 3' |
N1 |
5’-GAGAACCTGTACTTCCAGGGTGGTGCATCTACACGCGG TTTAATTAATCTTCTGCTAGCATGGTGAGCAAGGGCG-3’ |
N2 |
5’-GGGGACAAGTTTGTACAAAGCAGGCTCGGAGAACCTGTACTTCCAG-3’ |
C |
5’-GGGGACACACTTTGTACAAGAAAGCTGGGTTATTACTTGTA CAGCTCGTCCAT-3’ |
pDest-His6-MBP-FP sequencing FW |
5'-GATGAAGCCCTGAAAGACGCGCAG-3' |
pDest-His6-MBP-HIV-1 MA/CA P5-P4' FW |
5’-TAAAGTGAGCCAGAACTATCCGATTGTGCAGG-3’ |
pDest-His6-MBP-HIV-1 MA/CA P5-P4' REV |
5’-CTAGCCTGCACAATCGGATAGTTCTGGCTCACTTTAAT-3’ |
PR/IN 10aa forward |
5’- TAAAGTACCCACCATCAATAATGTCCATACAAGTG - 3’ |
PR/IN 10aa reverse |
5’- GATCCACTTGTATGGACATTATTGATGGTGGGTACTTTAAT - 3’ |
PR/IN 20aa forward |
5’- TAAACCATCAAATATCTCCGTACCCACCATCAATAATGTCCATACAAGTGAAAGT ACACGCAAAG - 3’ |
PR/IN 20aa reverse |
5’-GATCCTTTGCGTGTACTTTCACTTGTATGGACATTATTGATGGTGGGTACGGAGATATTTGATGGTTTAAT - 3’ |
PR/IN 20aa glycin forward |
5’- TAAAGGAGGCGGGGGTGGAGTACCCACCATCAATAATGTCCATACAAGTGGAGGC GGGGGTGGAG - 3’ |
PR/IN 20aa glycin reverse |
5’ - GATCCTCCACCCCCGCCTCCACTTGTATGGACATTATTGATGGTGGGTACTCC ACCCCCGCCTCCTTTAAT - 3’ |
IN/RT 10aa forward |
5’- TAAAATTCACCTGATTGCAGCTGTAAAAGCAGTAG - 3’ |
IN/RT 10aa reverse |
5’- GATCCTACTGCTTTTACAGCTGCAATCAGGTGAATTTTAAT - 3’ |
IN/RT 20aa forward |
5’- TAAAAGATCGAAGAAACGAATTCACCTGATTGCAGCTGTAAAAGCAGTAAAATCAATCAAACCAG - 3’ |
IN/RT 20aa reverse |
5’- GATCCTGGTTTGATTGATTTTACTGCTTTTACAGCTGCAATCAGGTGAATTCGTTTCTTCGATCTTTTAAT - 3’ |
GAG/PR 10aa forward |
5’- TAAAACAGCCAGGGCTCACAATGTATCCACATCTG - 3’ |
GAG/PR 10aa reverse |
5’- GATCCAGATGTGGATACATTGTGAGCCCTGGCTGTTTTAAT - 3’ |
GAG/PR 20aa forward |
5’-TAAAAATTCGAAATCGAAAACAGCCAGGGCTCACAATGTATCCACATCTAATAACTCTCCCAGCG - 3’ |
GAG/PR 20aa reverse |
5’- GATCCGCTGGGAGAGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAATTTTTAAT - 3’ |
Primer name |
Primer |
Ty1 PR forward |
5'-GCGCATATGAATGTATCCACATCTAATAACTCTCC-3 |
Ty1 PR reverse |
5'-CGCGGATCCATTGATGGTGGGTACGG-3' |
New-cloning cassette forward |
5’TAATCTTCTGGATCCGGTGGCGGCGGCTCAGGTGGTGGTGGCTCGGGTGGCGGCGGTTCGGGCGGTGGTGGCTCAG-3’ |
New-cloning cassette reverse |
5’CTAGCTGAGCCACCACCGCCCGAACCGCCGCCACCCGAGCCACCACCACCTGAGCCGCCGCCACCGGATCCAGAAGATTAAT-3’ |
sequencing forward primer |
5'-GATGAAGCCCTGAAAGACGCGCAG-3' |
Primer number |
Primer name |
Sequence |
1. |
PEG10 XhoI mutagenesis |
5′-gagctctcccacctggaggtcgccaagtcg-3′ |
2. |
PEG10 frameshift mutant primer |
5′-cttcgccggcgggaaaactccccggcccc-3′ |
3. |
RF1PEG10 and RF1/RF2PEG10 pQE-TriSystem forward- HindIII |
5′-gctcaagcttatgaccgaacgaagaagggac-3′ |
4. |
RF2PEG10 pQE-TriSystem forward- HindIII |
5’-gctcaagcttaaactccccggccccgctg-3′ |
5. |
RF1PEG10 pQE-TriSystem reverse1-Thrombin |
5′-ggatccacgcggaaccagcagcggggccggggagtttc-3′ |
6. |
RF2PEG10 and RF1/RF2PEG10 pQE-TriSystem reverse1- Thrombin |
5′-ggatccacgcggaaccaggtacgttgccaggtgtgc-3′ |
7. |
RF1PEG10, RF2PEG10 and RF1/RF2PEG10 pQE-TriSystem reverse2-XhoI |
5′-ctactcgagggatccacgcgaaccag-3′ |
8.a |
PRPEG10_D369A/D370A forward primer |
5′-ccgaggccatgatcgcttctggtg-3′ |
8.b |
PRPEG10_D369A/D370A reverse primer |
5′-ccagaagcaccagaagcgatcatggctcgg-3′ |
9.a |
PRPEG10_S371A forward primer |
5′-gagccatgatcgatgctggtgcttctggc-3′ |
9.b |
PRPEG10_S371A reverse primer |
5′-gccagaagcaccagcatcgatcatggctc-3′ |
10.a |
His6-MBP-fsRF1/RF2PEG10-mTurquoise2 forward primer |
5′-gcttaattaaaatgaccgagcgtcgtcgtg-3′ |
10.b |
His6-MBP-fsRF1/RF2PEG10-mTurquoise2 reverse primer |
5′-tagctagcgctcgcatcatcttgcggg-3′ |
Name |
Sequence |
forward-BamH1-SASP37 |
5’-G ATA GGA TCC GGG AGC CCA GGG GC-3’ |
forward-BamH1-SASP28 |
5’-G ATA GGA TCC GCC GGG AGC GGA GCC-3’ |
forward-BamH1-SASP14 |
5’-G ATA GGA TCC AGC ATC GGT AAG GGC-3’ |
reverse-EcoR1-SASP37/28 |
5’-CT ATT GAA TTC TCA GTG GGA TAG CTC CTG CCG-3’ |
reverse-EcoR1-SASP14 |
5’-CT ATT GAA TTC TCA CTC CAG GTC AAA CTC ATC TTC CAG-3’ |
SASP14-∆(K118-E136) |
5’-GCA CCC TGA AAG GGT AGA AGT TTC GCC TG-3’ |
SASP14-N103G/D107G/E109G |
5’-GAC CAC GGT GCT ATC CTG GGC TTT GGG CAC CGC ACA TGC-3’ |
SASP14-N103G |
5’-GAT GTG CTC CAG GAC CAC GGT GCT ATC CTG GGC TTT GGG-3’ |
SASP14-K116G |
5’-CGC ACA TGC ACC CTG GGA GGG AAG AAG TTT GGC-3’ |
SASP28-A189K/N190I |
5’-CTG CCC AAA GAG ATC GTC TTT AAG ATC AGC ATG GGT AAG GGC TAC-3’ |
SASP28-A189K/N190I |
5’-CTG CCC AAA GAG ATC GTC TTT AAG ATC AGC ATG GGT AAG GGC TAC-3’ |
SASP28-A167G/L168G |
5’-G GG ACT GTG AAA GAG GGC GGC CTG AAG GCC TTT GGG-3’ |